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1. IClip - Simply Useful Clipboard Manager iClip saves the contents of the clipboard every time you copy plain-text, rich-text, images, files, anything. Access your clip history in the gorgeous iClip interface. IClip for Mac OS X by Irradiated Software.
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iCLIP^[1] (individual-nucleotide resolution Cross-Linking and ImmunoPrecipitation) is a method used for identifying protein-RNA interactions. The method uses UV light to covalently bind proteins and RNA molecules. This cross-linking step has generally less background than RNA immunoprecipitation protocols.^[2][3] As with all CLIP methods, iCLIP allows for a very stringent purification of the linked protein-RNA complexes, using immunoprecipitation followed by SDS-PAGE and transfer to nitrocellulose. The radiolabelled protein-RNA complexes are then excised from nitrocellulose, and treated with proteinase to release the RNA, leaving one or two amino acids at the crosslink site of the RNA.

The RNA is then reverse transcribed, causing cDNAs to often truncate at the crosslink site, which is the key insight and unique feature in the development of iCLIP, as it allows identification of the site of RNA-protein interaction at high resolution. Analogous resolution can be obtained with standard HITS-CLIP methods, using the observation that RT that does read through the cross link site has a high error rate, which can be used to deduce the position of the crosslink ('Crosslink induced mutation site' (CIMS) analysis^[4]).

The small amount of resulting cDNAs is then PCR amplified and sequenced using a next-generation sequencing platform. The primer used for reverse transcription can contain a random sequence, which can be used to barcode cDNAs. This helps to identify PCR over-amplification effects in the high-throughput sequencing step and therefore improves the quantification of binding events.

References[edit]

1. ^König, Julian; Zarnack, Kathi; Luscombe, Nicholas M.; Ule, Jernej (18 January 2012). 'Protein–RNA interactions: new genomic technologies and perspectives'. Nature Reviews Genetics. 13 (2): 77–83. doi:10.1038/nrg3141. PMC4962561. PMID22251872.
2. ^Yao, Chengguo; Weng, Lingjie; Shi, Yongsheng (20 February 2014). 'Global protein-RNA interaction mapping at single nucleotide resolution by iCLIP-seq'. Methods in Molecular Biology. 1126: 399–410. doi:10.1007/978-1-62703-980-2_27. PMC4412157. PMID24549678.
3. ^Huppertz, Ina; Attig, Jan; D'Ambrogio, Andrea; Easton, Laura E; Sibley, Christopher R; Sugimoto, Yoichiro; Tajnik, Mojca; König, Julian; Ule, Jernej (February 2014). 'iCLIP: protein-RNA interactions at nucleotide resolution'. Methods. 65 (3): 274–87. doi:10.1016/j.ymeth.2013.10.011. PMC3988997. PMID24184352.
4. ^Zhang, Chaolin; Darnell, Robert B (1 June 2011). 'Mapping in vivo protein-RNA interactions at single-nucleotide resolution from HITS-CLIP data'. Nature Biotechnology. 29 (7): 607–614. doi:10.1038/nbt.1873. PMC3400429. PMID21633356.

Have you often copied something but when you tried to paste it you realized that you copied over it?

Do you ever want to copy multiple items before pasting them somewhere?

If you’re like most Mac users then the answer to these questions is a loud “yes!”

What you need is a supercharged clipboard that enables you to copy several times before you ever have to paste anything and that’s where iClip comes in.

Where the built-in Mac clipboard can only copy one thing at a time, iClip automatically stores a history of all the things you’ve copied.

You can copy…copy…copy then paste…paste…paste at your convenience. Can you see how useful iClip will be in your workflow?

If you value your time, you owe it to yourself to be the next Mac user to start saving time and become more productive with iClip.

One of the keys to iClip’s popularity is its simplicity and ease of use.

First off, iClip was designed to be accessed as simply as the Mac’s Dock is. Just move your cursor to the side of the screen and iClip pops out for instant use.

B&b others driver. To get things into and out of iClip you can…

• Drag & drop to and from your documents.
• Click on the arrows next to each bin to copy & paste to/from your documents with just 1-click.
• Go beyond the simple with powerful, customizable keyboard hot keys (shortcuts).

If you appreciate meticulous design and solid UI then you’ll love iClip.

Move the cursor over a clip bin for a moment to display a pop-up preview of the contents of a clip.

Imagine if ice cream came in only one flavor. Not much fun, eh? Take hold of the iClip preferences and go beyond plain vanilla.

• Choose the iClip screen position where it’s most convenient for the way you work.
• Try the various skins, bin shapes, and sizes.
• Adjust the transparency to your liking.
• and much, much more…

iClip isn’t just good looks. As simple as iClip is to use with your mouse, we didn’t forget the keyboard lovers in the crowd.

You’re able to use just about every iClip function with only your keyboard. Efficiently copy & paste in iClip without having to remove your hands from your keyboard.

And to make it even better, all of iClip’s keyboard shortcuts are fully customizable by you. Drivers argus over usb port devices.

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For when you’re repeating tasks, such as filling-out web forms, you can organize your stored clips into multiple sets and have them ready to paste in an instant.

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You can have a set of clips for each project you’re working on and store various things like web addresses, frequently used clip-art like logos, code snippets, etc.

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If you often have to respond to emails with a bunch of similar responses, you can use iClip to store them all. Having these all a single click or keyboard shortcut away make it really fast and efficient to handle tasks like this.

Do you need special handling of certain clipboard contents when they get copied into iClip, such as recording additional information or cleaning up the data? Do you want to add your own operations to modify select clippings, such as turning all their text to lowercase? iClip is scriptable in both AppleScript and JXA (Javascript for Applications). For more information, see iClip Scripting.